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KMID : 1145120170150020214
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Asian Journal of Beauty and Cosmetology
2017 Volume.15 No. 2 p.214 ~ p.222
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Evaluation of Curcuma longa L. Water Extracts as Beauty Food Materials in B16F10 and Human Skin Fibroblasts
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Ju Hyeong-Jin
Lee Seong-Ae
Kim Ryeong-Hyeon
Park Bu-Duk
Kim Gyo-Nam
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Abstract
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Purpose: To test whether water extracts of Curcuma longa L. (CLE) have the potential as beauty food materials, the inhibitory effects of CLE on melanin accumulation in B16F10 cells and regulatory effects of CLE on the skin fibril-related genes in human skin fibroblasts (HSFs) were examined in this study.
Methods: Thiazolyl blue tetrazolium bromide (MTT) assay was used to evaluate the cytotoxicity of CLE in B16F10 or HSFs. Inhibitory effects of CLE on the melanin accumulation were evaluated by B16F10-eluted melanin content and image analysis. The expressions of skin fibril-related genes such as procollagen1α1, procollagen1α2, and elastin in HSFs were evaluated by reverse transcription-polymerase chain reaction (RT-PCR) analysis.
Results: Based on the cytotoxicity results, the concentrations of CLE were confirmed under 400 μg/mL in both B16F10 and HSFs. The cytotoxicity of B16F10 cells with CLE for 24 h was confirmed and CLE up to 400 μg/mL were used. The CLE from 50 to 400 μg/mL significantly inhibited melanin accumulation in B16F10 cells. The HSFs treated with CLE showed up-regulated skin fibril-related genes.
Conclusion: Those results show that CLE has benefits with skin-whitening and up-regulation of skin fibril-related genes, which means it could be used as an ingredient in beauty foods and/or cosmetic materials.
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KEYWORD
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Curcuma longa L., Skin, Melanin, Elasticity, Beauty food
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